|In the two-stage cell transformation theory,
cancer cells first receive initiation, which is mainly caused
by DNA damage, and then promotion, which enhances transformation.
Murine Balb/c 3T3 cells are widely used for transformation
experiments because they lose contact inhibition ability
when transformed. Electrolyzed reduced water (ERW), which
is produced near a cathode during electrolysis of water,
is an alkaline drinking water that is beneficial to health.
ERW contains a high concentration of dissolved hydrogen and
scavenge reactive oxygen species (ROS), along with a small
amount of platinum (Pt) nanoparticles (Pt nps) derived from
Pt-coated titanium electrodes. Pt nps stably disperse in
aqueous solution for a long time, and convert hydrogen molecules
to active hydrogen (atomic hydrogen) that can scavenge ROS.
Therefore, ERW supplemented with synthesized Pt nps is a
model strong reduced water. This is the first report that
ERW supplemented with synthesized Pt nps strongly prevents
transformation of Balb/c 3T3 cells. ERW was prepared by electrolysis
of 0.002 M NaOH solution using a batch-type electrolysis
device. Balb/c 3T3 cells were treated with 3-methyl cholanthrene
(MCA) as an initiation substance, followed by treatment with
phorbol-12-myristate-13-acetate (PMA) as a promotion substance.
MCA/PMA-induced formation of a transformation focus was strongly
suppressed by ERW supplemented with Pt nps but not by ERW or
Pt nps individually. ERW supplemented with Pt nps suppressed
transformation at the promoter stage, not at initiation, suggesting
that ERW supplemented with Pt nps suppressed the PMA-induced
augmentation of intracellular ROS. ERW supplemented with Pt
nps is a potential new antioxidant against carcinogenesis.
[Abbreviations]ASA-2P:ascorbic acid 2-phosphate,
ERW：electrolyzed reduced water, FBS:fetal bovine
acid, MCA:3-methyl cholanthrene, MEM:minimum
essential medium, PMA:phorbol-12-myristate-13-acetate,
Pt nanoparticles:Pt nps, ROS:reactive oxygen