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PLOS ONE
(September 2017)
PLOS ONE
(February 2017)
Renal Replacement Therapy
(March 2016)
Environmental Control
In Biology
(July 2014)
Medical Gas Research
(December 2013)
Hemodial International
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PLoS ONE
(July 2012)
Cytotechnology
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Trends in Food Science and Technology
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BMC Proceedings
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Bioscience,
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Nephrology Dialysis Transplantation
(December 2010)
Cytotechnology
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Bioscience,
Biotechnology,
and Biochemistry
(October 2010)
Nephrology Dialysis Transplantation
(April 2010)
Nephrology Dialysis Transplantation
(February 2010)
Free Radical Research
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Nephron Clinical Practice
(April 2009)
Langmuir
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Biological & Pharmaceutical Bulletin
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Hemodialysis International
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Kidney International
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Cytotechnology
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Cytotechnology
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List of Papers and Conference Presentations on Electrolyzed Reduced Water
What is electrolyzed reduced water?
Environmental Control In Biology (2014) Abstract of paper published in July 2014
Effects of Deoxidized Nutrient Solution on Growth of Komatsuna (Brassica rapa var. perviridis) Plants

Electrochemically reduced water (ERW) is produced near a cathode during electrolysis and exhibits an alkaline pH, contains richly dissolved hydrogen, and contains a small amount of platinum nanoparticles. ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of hydrogen levels; however, there is no report verifying the role of dissolved hydrogen in ERW.

In this report, we clarify whether the responsive factor for antioxidative activity in ERW is dissolved hydrogen. The intracellular ROS scavenging activity of ERW and hydrogen-dissolved water was tested by both fluorescent stain method and immuno spin trapping assay. We confirm that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity, and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved hydrogen, but lost its activity when autoclaved. An oxygen radical absorbance capacity assay, the 2,2-diphenyl-1-picrylhydrazyl assay and chemiluminescence assay could not detect radical-scavenging activity in both ERW and hydrogen-dissolved water. These results indicate that ERW contains electrolysis-dependent hydrogen and an additional antioxidative factor predicted to be platinum nanoparticles.

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